WebBinding of BosR to the BS2 site in the rpoS promoter region. (a) Sequences of probes representing BS2. Underlined nucleotides represent the putative BosR box. (b) EMSAs. The concentration of BosR is designated above each lane. Probe names are indicated below the image. Bound DNA is denoted by arrowheads. HMC and LMC denote the high- and low … WebApr 16, 2024 · Nucleotides representing the BS2 site of the rpoS promoter was synthesized and labeled with digoxigenin. In a typical EMSA, 30 fmol labeled BS2 probe was incubated with varying amounts of BosR at 37°C for 30 min. Increasing concentrations of protein (10-, 20-, 50-, 100-, 200-, and 500-nM) are designated by black triangles, while the reactions ...
Bodies and Structures 2.0: Lenses
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