WebJan 8, 2024 · The RNAs were detected using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, USA). NBT and BCIP solutions (BBI, Canada) were used to detect the RNA. Prediction of genes targeted by miRNAs. The transcriptome sequences of the Ago2 complexes of nucleus and cytoplasm served as a database for miRNA target … WebDIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of …
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WebROCHE DIG-High Prime. MilliporeSigma …of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots. General description: Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one… WebDIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: • Southern blots • Northern blots • Dot blots free dating site for teenagers
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WebDigoxin Dig High Prime Dna Labeling, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results … WebDigoxigenin High Prime Dna Labeling Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebSouthern blot hybridization and the methods for DIG detec-tion were those recommended by the manufacturers of the membranes. In Southern blots, a full length RGA1 cDNA was labeled with DIG-High Prime (Boehringer Mannheim) and used as a probe. Mapping of the a-Subunit Gene on the Chromosome in Rice. All procedures were carried out according … blood red roses shanty