WebFeb 20, 2024 · Examine the smear with a fluorescence microscope and use the 10X objective to focus the smear. Finally, observe the smear using the 40 X objective for acid … Webpreparation and fixing smears, fluorochrome staining and reading acid-fast smears. Part II contains photomicrographs and descriptions of smears stained with various …

Acid Fast Bacilli (AFB) Smear and Culture Testing - UC Davis

WebLIMITATIONS OF PROCEDURES 1. Overheating (burning) during fixation should be avoided. 2. Do not stain smears which have only been air-dried. Smears must also be “fixed”. 3. Smears should not be too thick. After air-drying, examine under a microscope. 4. After staining it is essential that the back surface of the slide is wiped clean. 5. WebNov 28, 2024 · The gram staining procedure involves four major steps; staining with crystal violet, fixing the dye, applying a decolorizer, and counter-staining. Flood air-dried, heat-fixed smear of cells for 1 minute … how to scan qr code with citra

Flow cytometry introduction Abcam

WebGeneral Notes on FACS Staining: Store vials at 2 - 8°C in the dark. Do not freeze fluorochrome-conjugated antibodies. To ensure maximum recovery of antibody volume and proper mixing, quickly centrifuge the vial prior to use and use a pipette to mix the solution; do not vortex antibodies. Antibody-binding kinetics are temperature-dependent. WebRecommended Procedure 1. Place the slide containing the fixed smear onto a level staining rack. Ensure that you have access to distilled or deionized water for the rinsing process prior to proceeding. 2. Flood the slide with Auramine O Stain. Allow the smear to stain for 15 minutes. Ensure the stain stays on the smear. 3. WebFluorochrome Staining. Either small fluorochromes staining selectively mitochondria or fluorescent proteins are used. From: International Review of Cell and Molecular … north mymms park wedding