WebJan 1, 2024 · Human iPSC–derived intestinal epithelial cell monolayers (iPS-IECMs) were generated on the chamber. We examined whether these monolayers could be used to … WebDec 18, 2024 · Similarly, IL-2 derived from activated T cells enhanced the growth of human iPSC-derived intestinal organoids, as well as the expression of markers of mature intestinal epithelial cells ...
Generation of Human-Induced Pluripotent Stem Cell-Derived
WebAs iPSC-derived HIOs contain epithelial and mesenchymal cells, the organoids were dissociated to generate purified cultures of epithelial-only HIOs (eHIOs) and … WebMar 31, 2024 · The gut barrier and BBB are described, an overview of current cell models are provided, and the use of iPSCs in these GBA elements are discussed, highlighting the perspectives of producing GBA chips usingiPSCs and the challenges that remain in the field. The gut microbiome (GM), the gut barrier, and the blood-brain barrier (BBB) are key … potassium too high diet
Human Intestinal Organoids, iPSC Derived Products
WebApr 1, 2024 · In a different approach, iPSC-derived intestinal organoids can be established by differentiating iPSCs into definitive endoderm by using activin A and the addition of Wnt3A and FGF2, ... A refined culture system for human induced pluripotent stem cell-derived intestinal epithelial organoids. Stem Cell Rep. 2024;10(1):314–28. Gene expression profiles of IEC layers exposed to the three individual SCFAs at two concentrations were evaluated by a PCA. A PCA scatterplot representing the first two principle components based on the transcriptome profiles of the six exposure groups and the controls is shown in Fig. 1. PC1 and PC2 explain 54.71% … See more The cellular makeup of the IEC layers was characterized by evaluating the expression of known cell type specific gene markers in the control samples. This was … See more The original dataset consisted of 60,237 genes in 21 samples, after filtering for low count genes this was reduced to 19,654 genes. Of these genes, 3,534 were not … See more To evaluate the effects of the SCFAs on differentiation of IECs, gene expression of specific differentiation and cell type markers was analysed in all exposure … See more WebNumerous cell types, including intestinal epithelial and mesenchymal cells, are implicated in this but research efforts are impaired by a lack of in vitro models. Human intestinal organoids (HIOs), derived from induced pluripotent stem cells (iPSCs), are comprised of both of these cell types; therefore iPSC-derived HIOs represent an approach in ... potassium too high in blood