Phospholipidosis detection reagent
WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ∼595/615 nm. WebOct 30, 2024 · In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma …
Phospholipidosis detection reagent
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WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar … WebLipidTox Green neutral lipid stain and LipidTox Red phospholipidosis detection reagent (Kit from Molecular Probes) ... ORIGINAL ARTICLE 232 E-LDL and Ox-LDL Differ in the Lipid Storage. phospholipidosis detection depending on the emission spectra of the dye (Table 1) (30,37–39). Lipid-loaded macrophage foam cells play a major role in
WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar bodies visible under an electron microscope (EM). 1 Drugs, including many antibiotics, antidepressants, and antihistamines, induce PLD in various tissues such as lung, 2 heart, …
WebH34157 HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit *for high content screening* *for cellular imaging* *2-plate size*. . . . . 1 kit ... H34350 HCS LipidTOX™ Green phospholipidosis detection reagent *1000X aqueous solution* *for cellular imaging* 10-plate size* . . . . . . . . . each WebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their …
WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is …
WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … body shop in oklahoma cityWebDec 7, 2024 · Annexin V-FITC/PI apoptosis detection kit was acquired from BD (Franklin Lakes, NJ, USA). Then, Hoechst 33342, eBioscience™ JC-1 Mitochondrial Membrane Potential Dye, Cell ROX Deep Red, and HCS LipidTOX™ Green Phospholipidosis Detection Reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA). glens pool fencingWebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima approx. 595/615nm ... glen spooner observing chair earthshineWebInvitrogen’s HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kits offer the reagents for performing image-based high-content screening (HCS) assays to … body shop in orlando flWebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological … glens small bottleWebInvitrogen™ HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging. Easily detected by fluorescence microscopy. Manufacturer: Invitrogen™ … glens reach laugharneWebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and ... glen spey ny map