Rk2 replicon
WebJul 1, 2009 · RK2 replicons potentially eliminate these types of limitations, and large-sized poorly characterized cosmid-RK2-derivatives have been used for metagenomic library … WebThe RK2 replicon plasmids produced a level of ir that was equivalent to the wild-type level (pMM4), or 2.25-fold (pPR1A6R1), 4.5-fold (pPR1A6R3), or 9.0-fold (pPR1A6) above …
Rk2 replicon
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Webthe narrow host range replicon oriR6K, the sacB gene and a chloramphenicol resistance gene, using the pir-expressing E. coli strain S17-1lpir as host. The deletions within RK2 … WebThe minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-acting replication), encoding a protein required for initiation of plasmid replication. The …
WebThe 393-base pair minimal origin,oriV, of plasmid RK2 contains three iterated motifs essential for initiation of replication: consensus sequences for binding the bacterial DnaA … WebVector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not …
WebApr 5, 2016 · To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of … <i>
WebThe binary vector pPCV001 (9.2 kbp) has a conditional mini-RK2 replicon (oriV and oriT) and can be maintained in Agrobacterium and E. coli only when co-existed in trans with the trf …
WebKeywords. trfA deletion; oriV RK2 replicon. Introduction Maintenance of a plasmid in a given bacterial host largely depends upon its replication and/or separation proficiency. Among … hrcr hobbiesWebBroad-host-range plasmid RK2-based promoter probe vectors with a known nucleotide sequence were constructed. In the absence of an upstream promoter, the expression of … hr crinkleWebJul 1, 1997 · This report describes the construction and use of improved broad-host-range expression vectors based on the previously constructed pJB137 and pJB653 plasmids … hrc rgd-111WebApr 11, 2024 · In order to slowly approach (and not to exceed) the correct ATPase level, its expression was driven from a low-copy broad-host range (RK2 replicon) vector (Blatny et al., 1997a, 1997b) in the E. coli K-12 MG1655 wild-type strain. hrc rmpdWebSpecifically, based on the broad host range RK2 replicon, we have developed a new, relatively small and well-known vector. Such vectors are useful for the construction of … hrcr navigation handbookWebNov 1, 2013 · Name of replicon Name of vector constructed Type of vector Antibiotic resistance Size (kb) Reference RK2 pRK290 Cloning vector Tc 20 Ditta et al. (1985) RK2 … hrcr open day 2022WebJul 20, 2015 · Here, using the plasmid RK2 replicon, we analyze the protein interactions required for Escherichia coli polymerase III (Pol III) holoenzyme association at the … hrcr membership