WebThe single stranded DNA (ssDNA) with G-rich sequence can fold into G-quadruplex via intramolecular hydrogen-bonding interaction in the presence of ligand. This structure conversion can be specifically detected by a fluorescence method based on different interaction between SYBR Green I (SG) and various DNA structures. WebApplied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and SYBR Green dye—everything needed for real-time PCR. Find the best master mix by application.
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WebJul 28, 2011 · Background Real-time PCR can be carried out using either probes or DNA dyes. SYBR Green has been used the most, but it suffers from several drawbacks. Numerous other DNA dyes are commercially available, but with limited structural information. Dye behavior in real time PCR is difficult to predict, so empirical data are … WebThe detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property relationships that affect methods applying SG. These … エッグスタンド 動物
Optimizing the SYBR green related cyanine dye structure to aim …
WebApr 2, 2015 · Science. qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt ... WebThe distortion in the local DNA structure caused by YOYO-1 bis-intercalation has been observed by two-dimensional NMR spectroscopy. At high dye:base pair ratios, ... and the SYBR Green I stain has been shown to be significantly less mutagenic than EtBr by Ames testing (Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4). WebSYBR Green I is the most frequently used dsDNA-specific dye in qPCR. It is an asymmetric cyanine dye that largely binds to the minor groove of dsDNA, independent of the … エックスネット